Bioscientifica Proceedings

The reconstruction of mammalian embryos by transfer of a blastomere nucleus to an enucleated oocyte or zygote allows for the production of genetically identical individuals. This has advantages for research (that is, as biological controls) and commercial applications (that is, multiplication of genetically valuable livestock). However, the number of offspring that can be produced from a single embryo is limited both by the number of blastomeres (embryos at the 32–64-cell...

In nuclear transfer reconstructed embryos, the co-ordination of donor nuclear and recipient cytoplasmic cell cycle phases is essential to maintain ploidy and prevent DNA damage. However, the stage of the cell cycle at the time of reconstruction and the method of reconstruction may also have a significant impact on the subsequent development of the embryo and fetus through a number of other mechanisms. This paper reviews some of the information currently available and proposes ...